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V5-Tag Mouse mAb

一鍵復(fù)制產(chǎn)品信息

EM1004

規(guī)格：	價(jià)格：
50μL	￥1280.00
100μL	￥1980.00

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Overview

Product name:	V5-Tag Mouse mAb
Source:	Mouse
Dilutions:	WB 1:5,000 IP 1:200 IF 1:1,000
Immunogen:	Synthetic Peptide
Storage:	PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid?repeated?freeze-thaw?cycles.
Clonality:	Monoclonal
Isotype:	IgG1
Concentration:	1mg/mL
Background:	The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag antibody can be helpful in detecting the recombinant proteins, some of which include transmembrane and secreted proteins fusion protein.

IP antibody use：5ug V5 Mouse IgG1 per ml Lysate，WB 1:5000 1、untransfected 293 cell lysate 2、transfected 293 cell lysate with V5-tag fusion protein 3、IP(untransfted 293+anti-V5 mAb+Protein G agarose) 4、IP (transfected 293+ normal Mouse IgG+Protein G ag
IF analysis of 293 cells transfected with a V5-tag protein, using ELKbio anti-V5Tag Mouse mAb at a 1:2000 dilution (blue DAPI, red anti-V5)
1ug V5 fusion protein+ Primary antibody dilution at 1、1:5,000 2、1:10,000

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