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產(chǎn)品中心

His-tag rabbit pAb

一鍵復(fù)制產(chǎn)品信息

ES1076

規(guī)格：	價(jià)格：
50μL	￥1280.00
100μL	￥1980.00

下載說(shuō)明 ①

Overview

Product name:	His-tag rabbit pAb
Reactivity:	Species independent
Alternative Names:	6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag; His tag
Source:	Rabbit
Dilutions:	Western Blot: 1/1000 - 1/3000. ELISA: 1/20000. Not yet tested in other applications.
Immunogen:	6X His synthetic peptide conjugated to KLH.
Storage:	-20°C/1 year
Clonality:	Polyclonal
Isotype:	IgG
Concentration:	1 mg/ml
Observed Band:	15kD
Background:	The his-tag is a series of six to nine histidine residues generally fused to either the carboxy or amino terminus of a recombinant protein. The small size of the his-tag, compared with other common epitope tags, makes it less likely to obstruct the target protein’s structure or function and more suitable to use under denaturing conditions. The string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper. The binding to metal ions under specific buffer conditions, allows for the simple purification and detection of his-tagged proteins.

Western Blot analysis using His-tag Polyclonal Antibody against HEK293 cells transfected with vector overexpressing His tag (1) and untransfected (2). Antibody was diluted at 1:2000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
Western Blot analysis of HIS-protein cells using His-tag Polyclonal Antibody diluted at 1:2000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000

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